Immunocytochemical staining of HAP-1 cells with Chromogranin A antibody (Cat#63560, 1:1,000) . Nuclei were stained blue with DAPI; Chromogranin A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-chromogranin A antibody (Cat#63560). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-chromogranin A antibody (Cat#63560, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-chromogranin A antibody (Cat#63560). Chromogranin A expression in wild type (WT) and chromogranin A (CHGA) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-chromogranin A antibody (Cat#63560, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
