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KD-Validated Anti-SERPINB1 Mouse Monoclonal Antibody #63564

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Flow cytometric analysis of Serpin family B member 1 expression in HepG2 cells using anti-Serpin family B member 1 antibody (Cat#63564, 1:2,000). Green, isotype control; red, Serpin family B member 1.
Western blotting analysis using anti-serpin family B member 1 antibody (Cat#63564). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-serpin family B member 1 antibody (Cat#63564, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-serpin family B member 1 antibody (Cat#63564). Serpin family B member 1 expression in wild type (WT) and serpin family B member 1 (SERPINB1) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-serpin family B member 1 antibody (Cat#63564, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    SERPINB1
  • 货号:
    63564
  • 应用:
    WB, FCM
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KD-Validated Anti-SERPINB1 Mouse Monoclonal Antibody
Aliases SERPINB1; Serpin Family B Member 1; MNEI; PI2; LEI; EI; Leukocyte Elastase Inhibitor; ELANH2; Serine (Or Cysteine) Proteinase Inhibitor, Clade B (Ovalbumin), Member 1; Serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 1; Monocyte/Neutrophil Elastase Inhibitor; Peptidase Inhibitor; M/NEI; PI-2; Protease Inhibitor 2 (Anti-Elastase), Monocyte/Neutrophil Derived; Epididymis Secretory Protein Li 27; Epididymis Luminal Protein 57; Anti-Elastase; Serpin B1; HEL-S-27; HEL57
Background

Gene Name: SERPINB1

NCBI Gene Entry: 1992

UniProt Entry: P30740

Application Information

Molecular Weight: Predicted, 43 kDa; observed, 43 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB6540

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen Recombinant protein of human SERPINB1
Isotype Mouse IgG2b
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Serpin family B member 1 expression in HepG2 cells using anti-Serpin family B member 1 antibody (Cat#63564, 1:2,000). Green, isotype control; red, Serpin family B member 1.
  • Western blotting analysis using anti-serpin family B member 1 antibody (Cat#63564). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-serpin family B member 1 antibody (Cat#63564, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-serpin family B member 1 antibody (Cat#63564). Serpin family B member 1 expression in wild type (WT) and serpin family B member 1 (SERPINB1) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-serpin family B member 1 antibody (Cat#63564, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of Serpin family B member 1 expression in HepG2 cells using anti-Serpin family B member 1 antibody (Cat#63564, 1:2,000). Green, isotype control; red, Serpin family B member 1.
Western blotting analysis using anti-serpin family B member 1 antibody (Cat#63564). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-serpin family B member 1 antibody (Cat#63564, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-serpin family B member 1 antibody (Cat#63564). Serpin family B member 1 expression in wild type (WT) and serpin family B member 1 (SERPINB1) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-serpin family B member 1 antibody (Cat#63564, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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