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KD-Validated Anti-CHCHD3 Mouse Monoclonal Antibody #63568

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Western blotting analysis using anti-CHCHD3 antibody (Cat#63568). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CHCHD3 antibody (Cat#63568, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Western blotting analysis using anti-CHCHD3 antibody (Cat#63568). CHCHD3 expression in wild type (WT) and CHCHD3 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CHCHD3 antibody (Cat#63568, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Immunocytochemical staining of HeLa cells using anti-CHCHD3 antibody (Cat#63568, 1:1,000), Top panel: wild-type (WT); Bottom panal: Adiponectin receptor 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; CHCHD3 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

基因名：

CHCHD3
货号：

63568
应用：

WB, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information

Product Name	KD-Validated Anti-CHCHD3 Mouse Monoclonal Antibody
Aliases	CHCHD3; Coiled-Coil-Helix-Coiled-Coil-Helix Domain Containing 3; MINOS3; PPP1R22; MICOS19; Mic19; Mitochondrial Contact Site And Cristae Organizing System Subunit 19; Mitochondrial Inner Membrane Organizing System 3; Protein Phosphatase 1, Regulatory Subunit 22; MICOS Complex Subunit MIC19; FLJ20420; Coiled-Coil-Helix-Coiled-Coil-Helix Domain-Containing Protein 3, Mitochondrial; Coiled-Coil-Helix-Coiled-Coil-Helix Domain-Containing Protein 3; MIC19
Background	Gene Name: CHCHD3 NCBI Gene Entry: 54927 UniProt Entry: Q9NX63
Application Information	Molecular Weight: Predicted, 26 kDa; observed, 26 kDa Clonality: Mouse monoclonal antibody Clone ID: 24GB6560 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), immunocytochemistry (IC)
Immunogen	Recombinant protein of human CHCHD3
Isotype	Mouse IgG1
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Western blotting analysis using anti-CHCHD3 antibody (Cat#63568). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CHCHD3 antibody (Cat#63568, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-CHCHD3 antibody (Cat#63568). CHCHD3 expression in wild type (WT) and CHCHD3 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CHCHD3 antibody (Cat#63568, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HeLa cells using anti-CHCHD3 antibody (Cat#63568, 1:1,000), Top panel: wild-type (WT); Bottom panal: Adiponectin receptor 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; CHCHD3 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

Citation(0)

基因名：

CHCHD3
货号：

63568
应用：

WB, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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