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KD-Validated Anti-EHMT2 Rabbit Monoclonal Antibody #68352

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Flow cytometric analysis of EHMT2 expression in HepG2 cells using EHMT2 antibody (Cat#68352, 1:2,000). Green, isotype control; red, EHMT2 .
Immunocytochemical staining of HepG2 cells with EHMT2 antibody (Cat#68352, 1:1,000). Nuclei were stained blue with DAPI; EHMT2 was stained magenta with Alexa Fluor® 647. Scale bar: 20 μm.
Western blotting analysis using anti-EHMT2 antibody (Cat#68352). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EHMT2 antibody (Cat#68352, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-EHMT2 antibody (Cat#68352). EHMT2 expression in wild type (WT) and EHMT2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-EHMT2 antibody (Cat#68352, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of EHMT2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-EHMT2 antibody (Cat#68352, 1:2,000) and analyzed using CytoFLEX.
Immunocytochemical staining of HeLa cells using anti-EHMT2 antibody (Cat#68352, 1:1,000), Top panel: wild-type (WT); Bottom panal: EHMT2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; EHMT2 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
  • 基因名：
    EHMT2
  • 货号：
    68352
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-EHMT2 Rabbit Monoclonal Antibody
Aliases Euchromatic Histone Lysine Methyltransferase 2; KMT1C; G9A; C6orf30; BAT8; Euchromatic Histone-Lysine N-Methyltransferase 2; Histone-Lysine N-Methyltransferase EHMT2; Histone H3-K9 Methyltransferase 3; HLA-B Associated Transcript 8; Lysine N-Methyltransferase 1C; H3-K9-HMTase 3; Em:AF134726.3; NG36/G9a; NG36; Histone-Lysine N-Methyltransferase, H3 Lysine-9 Specific 3; Chromosome 6 Open Reading Frame 30; Ankyrin Repeat-Containing Protein; G9A Histone Methyltransferase; HLA-B-Associated Transcript 8; EC 2.1.1.367; Protein G9a; EC 2.1.1.-; GAT8
Background

Gene Name: EHMT2

NCBI Gene Entry: 10919

UniProt Entry: Q96KQ7
Application Information

Molecular Weight: Predicted, 132 kDa, observed, 160-170 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB825

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human EHMT2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of EHMT2 expression in HepG2 cells using EHMT2 antibody (Cat#68352, 1:2,000). Green, isotype control; red, EHMT2 .
  • Immunocytochemical staining of HepG2 cells with EHMT2 antibody (Cat#68352, 1:1,000). Nuclei were stained blue with DAPI; EHMT2 was stained magenta with Alexa Fluor® 647. Scale bar: 20 μm.
  • Western blotting analysis using anti-EHMT2 antibody (Cat#68352). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EHMT2 antibody (Cat#68352, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-EHMT2 antibody (Cat#68352). EHMT2 expression in wild type (WT) and EHMT2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-EHMT2 antibody (Cat#68352, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Validation of EHMT2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-EHMT2 antibody (Cat#68352, 1:2,000) and analyzed using CytoFLEX.
  • Immunocytochemical staining of HeLa cells using anti-EHMT2 antibody (Cat#68352, 1:1,000), Top panel: wild-type (WT); Bottom panal: EHMT2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; EHMT2 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
Citation(0)
  • 基因名：
    EHMT2
  • 货号：
    68352
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of EHMT2 expression in HepG2 cells using EHMT2 antibody (Cat#68352, 1:2,000). Green, isotype control; red, EHMT2 .
Immunocytochemical staining of HepG2 cells with EHMT2 antibody (Cat#68352, 1:1,000). Nuclei were stained blue with DAPI; EHMT2 was stained magenta with Alexa Fluor® 647. Scale bar: 20 μm.
Western blotting analysis using anti-EHMT2 antibody (Cat#68352). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EHMT2 antibody (Cat#68352, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-EHMT2 antibody (Cat#68352). EHMT2 expression in wild type (WT) and EHMT2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-EHMT2 antibody (Cat#68352, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of EHMT2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-EHMT2 antibody (Cat#68352, 1:2,000) and analyzed using CytoFLEX.
Immunocytochemical staining of HeLa cells using anti-EHMT2 antibody (Cat#68352, 1:1,000), Top panel: wild-type (WT); Bottom panal: EHMT2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; EHMT2 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
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