Flow cytometric analysis of HNRNPA0 expression in HepG2 cells using anti-HNRNPA0 antibody (Cat#63580, 1:2,000). Green, isotype control; red, HNRNPA0.
Immunocytochemical staining of HepG2 cells with anti-HNRNPA0 antibody (Cat#63580, 1:1,000) . Nuclei were stained blue with DAPI; HNRNPA0 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：Medium. Scale bar, 20 μm.
Western blotting analysis using anti-HNRNPA0 antibody (Cat#63580). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HNRNPA0 antibody (Cat#63580, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-HNRNPA0 antibody (Cat#63580). HNRNPA0 expression in wild type (WT) and HNRNPA0 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-HNRNPA0 antibody (Cat#63580, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HeLa cells using anti-HNRNPA0 antibody (Cat#63580, 1:1,000), Top panel: wild-type (WT); Bottom panal: HNRNPA0 shRNA knockdown (KD). Nuclei were stained blue with DAPI; HNRNPA0 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
