Immunocytochemical staining of C2C12 cells with anti-Adenylate kinase 3 antibody (Cat#63638, 1:1,000) . Nuclei were stained blue with DAPI; Adenylate kinase 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-adenylate kinase 3 antibody (Cat#63638). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-adenylate kinase 3 antibody (Cat#63638, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-adenylate kinase 3 antibody (Cat#63638). Adenylate kinase 3 expression in wild type (WT) and adenylate kinase 3 (AK3) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-adenylate kinase 3 antibody (Cat#63638, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of Adenylate kinase 3 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Adenylate kinase 3 antibody (Cat#63638, 1:2,000) and analyzed using BD flow cytometer.
