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KD-Validated Anti-PSMB8 Mouse Monoclonal Antibody #63657

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Western blotting analysis using anti-proteasome 20S subunit beta 8 antibody (Cat#63657). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-proteasome 20S subunit beta 8 antibody (Cat#63657, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-proteasome 20S subunit beta 8 antibody (Cat#63657). Proteasome 20S subunit beta 8 expression in wild-type (WT) and proteasome 20S subunit beta 8 (PSMB8) shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-proteasome 20S subunit beta 8 antibody (Cat#63657, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of Proteasome 20S subunit beta 8 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HT-1080 cells were stained with anti-Proteasome 20S subunit beta 8 antibody (Cat#63657, 1:2,000) and analyzed using BD flow cytometer.
  • 基因名：
    PSMB8
  • 货号：
    63657
  • 应用：
    WB, FCM
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-PSMB8 Mouse Monoclonal Antibody
Aliases PSMB8; Proteasome 20S Subunit Beta 8; RING10; PSMB5i; D6S216E; LMP7; Multicatalytic Endopeptidase Complex Subunit C13; Really Interesting New Gene 10 Protein; Proteasome Subunit Beta Type-8; Low Molecular Mass Protein 7; Proteasome Subunit Beta 8; Proteasome Component C13; Macropain Subunit C13; EC 3.4.25.1; Beta5i; Proteasome (Prosome, Macropain) Subunit, Beta Type, 8 (Large Multifunctional Peptidase 7); Proteasome (Prosome, Macropain) Subunit, Beta Type, 8 (Large Multifunctional Protease 7); Proteasome (Prosome, Macropain) Subunit, Beta Type, 8; Large Multifunctional Peptidase 7; Proteasome Catalytic Subunit 3i; Low Molecular Weight Protein 7; Proteasome Subunit Beta 5i; Proteasome Subunit Beta-5i; Proteasome-Related Gene 7; Proteasome Subunit Β5i; Protease Component C13; Proteasome Subunit Y2; D6S216; PRAAS1; ALDD; NKJO; JMP; Y2
Background

Gene Name: PSMB8

NCBI Gene Entry: 5696

UniProt Entry: P28062
Application Information

Molecular Weight: Predicted, 30 kDa; observed, 23 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB6765

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen Recombinant protein of human PSMB8
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-proteasome 20S subunit beta 8 antibody (Cat#63657). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-proteasome 20S subunit beta 8 antibody (Cat#63657, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-proteasome 20S subunit beta 8 antibody (Cat#63657). Proteasome 20S subunit beta 8 expression in wild-type (WT) and proteasome 20S subunit beta 8 (PSMB8) shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-proteasome 20S subunit beta 8 antibody (Cat#63657, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Validation of Proteasome 20S subunit beta 8 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HT-1080 cells were stained with anti-Proteasome 20S subunit beta 8 antibody (Cat#63657, 1:2,000) and analyzed using BD flow cytometer.
Citation(0)
  • 基因名：
    PSMB8
  • 货号：
    63657
  • 应用：
    WB, FCM
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Western blotting analysis using anti-proteasome 20S subunit beta 8 antibody (Cat#63657). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-proteasome 20S subunit beta 8 antibody (Cat#63657, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-proteasome 20S subunit beta 8 antibody (Cat#63657). Proteasome 20S subunit beta 8 expression in wild-type (WT) and proteasome 20S subunit beta 8 (PSMB8) shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-proteasome 20S subunit beta 8 antibody (Cat#63657, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of Proteasome 20S subunit beta 8 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HT-1080 cells were stained with anti-Proteasome 20S subunit beta 8 antibody (Cat#63657, 1:2,000) and analyzed using BD flow cytometer.
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