Flow cytometric analysis of Annexin A1 expression in HepG2 cells using anti-Annexin A1 antibody (Cat#63667, 1:2,000). Green, isotype control; red, Annexin A1.
Immunocytochemical staining of HepG2 cells with anti-Annexin A1 antibody (Cat#63667, 1:1,000) . Nuclei were stained blue with DAPI; Annexin A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-annexin A1 antibody (Cat#63667). Total cell lysates (10 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-annexin A1 antibody (Cat#63667, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-annexin A1 antibody (Cat#63667). Annexin A1 expression in wild-type (WT) and annexin A1 (ANXA1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-annexin A1 antibody (Cat#63667, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of Annexin A1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Annexin A1 antibody (Cat#63667, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of Hela cells using anti-Annexin A1 antibody (Cat#63667, 1:1,000), Top panel: wild-type (WT); Bottom panal: Annexin A1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Annexin A1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
