Flow cytometric analysis of PPP2R5D expression in HepG2 cells using anti-PPP2R5D antibody (Cat#61681, 1:2,000). Green, isotype control; red, PPP2R5D.
Immunocytochemical staining of PPP2R5D cells with PPP2R5D antibody (Cat#61681, 1:1,000). Nuclei were stained blue with DAPI; PPP2R5D was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-PPP2R5D antibody (Cat#61681). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PPP2R5D antibody (Cat#61681, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-PPP2R5D antibody (Cat#61681). PPP2R5D expression in wild type (WT) and PPP2R5D knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PPP2R5D antibody (Cat#61681, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
