Immunocytochemical staining of C2C12 cells with anti-Annexin A6 antibody (Cat#63669, 1:1,000) . Nuclei were stained blue with DAPI; Annexin A6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-annexin A6 antibody (Cat#63669). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-annexin A6 antibody (Cat#63669, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-annexin A6 antibody (Cat#63669). Annexin A6 expression in wild-type (WT) and annexin A6 (ANXA6) shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-annexin A6 antibody (Cat#63669, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of Annexin A6 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Annexin A6 antibody (Cat#63669, 1:2,000) and analyzed using BD flow cytometer.
