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KD-Validated Anti-MAPK3 Mouse Monoclonal Antibody #63710

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Western blotting analysis using anti-ERK1 antibody (Cat#63710). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ERK1 antibody (Cat#63710, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using anti-ERK1 antibody (Cat#63710). ERK1 expression in wild-type (WT) and MAPK3 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ERK1 antibody (Cat#63710, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Validation of ERK1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-ERK1 antibody (Cat#63710, 1:2,000) and analyzed using BD flow cytometer.

基因名：

MAPK3
货号：

63710
应用：

WB, FCM
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

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Information

Product Name	KD-Validated Anti-MAPK3 Mouse Monoclonal Antibody
Aliases	MAPK3; Mitogen-Activated Protein Kinase 3; ERK1; P44mapk; P44erk1; PRKM3; Extracellular Signal-Regulated Kinase 1; Microtubule-Associated Protein 2 Kinase; Insulin-Stimulated MAP2 Kinase; EC 2.7.11.24; P44-ERK1; P44-MAPK; ERK-1; ERT2; Extracellular Signal-Related Kinase 1; MAP Kinase Isoform P44; MAP Kinase 3; EC 2.7.11; HS44KDAP; HUMKER1A; MAPK 1; MAPK 3
Background	Gene Name: MAPK3 NCBI Gene Entry: 5595 UniProt Entry: P27361
Application Information	Molecular Weight: Predicted, 43 kDa; observed, 37 kDa Clonality: Mouse monoclonal antibody Clone ID: 24GB7025 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human ERK1
Isotype	Mouse IgG1 kappa
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000
Note	This product is for research use only.

Picture

Western blotting analysis using anti-ERK1 antibody (Cat#63710). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ERK1 antibody (Cat#63710, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-ERK1 antibody (Cat#63710). ERK1 expression in wild-type (WT) and MAPK3 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ERK1 antibody (Cat#63710, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of ERK1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-ERK1 antibody (Cat#63710, 1:2,000) and analyzed using BD flow cytometer.

Citation(0)

基因名：

MAPK3
货号：

63710
应用：

WB, FCM
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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