KD-Validated Anti-FLT1 Mouse Monoclonal Antibody - 产品中心

KD-Validated Anti-FLT1 Mouse Monoclonal Antibody #63728

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Flow cytometric analysis of FLT1 expression in HepG2 cells using anti-FLT1 antibody (Cat#63728, 1:2,000). Green, isotype control; red, FLT1.

Immunocytochemical staining of HepG2 cells with anti-FLT1 antibody(Cat#63728, 1:1,000) . Nuclei were stained blue with DAPI; FLT1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using anti-FLT1 antibody (Cat#63728). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FLT1 antibody (Cat#63728, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). FLT1, fms related receptor tyrosine kinase 1.

Western blotting analysis using anti-FLT1 antibody (Cat#63728). FLT1 expression in wild-type (WT) and FLT1 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-FLT1 antibody (Cat#63728, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

FLT1
货号：

63728
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information

Product Name	KD-Validated Anti-FLT1 Mouse Monoclonal Antibody
Aliases	FLT1; Fms Related Receptor Tyrosine Kinase 1; VEGFR1; Vascular Endothelial Growth Factor Receptor 1; Vascular Permeability Factor Receptor; FLT; Fms-Related Tyrosine Kinase 1 (Vascular Endothelial Growth Factor/Vascular Permeability Factor Receptor); Tyrosine-Protein Kinase Receptor FLT; Fms Related Tyrosine Kinase 1; Tyrosine-Protein Kinase FRT; Fms-Like Tyrosine Kinase 1; EC 2.7.10.1; VEGFR-1; FLT-1; Fms-Related Tyrosine Kinase 1; EC 2.7.10; FRT
Background	Gene Name: FLT1 NCBI Gene Entry: 2321 UniProt Entry: P17948
Application Information	Molecular Weight: Predicted, 151 kDa; observed, 53 kDa Clonality: Mouse monoclonal antibody Clone ID: 24GB7115 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	Recombinant protein of human VEGFR1
Isotype	Mouse IgG1 kappa
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of FLT1 expression in HepG2 cells using anti-FLT1 antibody (Cat#63728, 1:2,000). Green, isotype control; red, FLT1.
Immunocytochemical staining of HepG2 cells with anti-FLT1 antibody(Cat#63728, 1:1,000) . Nuclei were stained blue with DAPI; FLT1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-FLT1 antibody (Cat#63728). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FLT1 antibody (Cat#63728, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). FLT1, fms related receptor tyrosine kinase 1.
Western blotting analysis using anti-FLT1 antibody (Cat#63728). FLT1 expression in wild-type (WT) and FLT1 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-FLT1 antibody (Cat#63728, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

FLT1
货号：

63728
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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