Western blotting analysis using anti-X-linked inhibitor of apoptosis antibody (Cat#63773). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-X-linked inhibitor of apoptosis antibody (Cat#63773, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-X-linked inhibitor of apoptosis antibody (Cat#63773). X-linked inhibitor of apoptosis expression in wild-type (WT) and X-linked inhibitor of apoptosis (XIAP) shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-X-linked inhibitor of apoptosis antibody (Cat#63773, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of X-linked inhibitor of apoptosis knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HT-1080 cells were stained with anti-X-linked inhibitor of apoptosis antibody (Cat#63773, 1:2,000) and analyzed using BD flow cytometer.
