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KD-Validated Anti-IMMT Mouse Monoclonal Antibody #65108

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Flow cytometric analysis of IMMT expression in HepG2 cells using anti-IMMT antibody (Cat#65108, 1:1,000) . Green, isotype control; red, IMMT.

Immunocytochemical staining of HepG2 cells with anti-IMMT antibody (Cat#65108, 1:1,000) . Nuclei were stained blue with DAPI; IMMT was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using anti-IMMT antibody (Cat#65108). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-IMMT antibody (Cat#65108, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Western blotting analysis using anti-IMMT antibody (Cat#65108). IMMT expression in wild-type (WT) and IMMT shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-IMMT antibody (Cat#65108, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

IMMT
货号：

65108
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information

Product Name	KD-Validated Anti-IMMT Mouse Monoclonal Antibody
Aliases	IMMT; Inner Membrane Mitochondrial Protein; MINOS2; HMP; Mitofilin; MICOS60; Mic60; P87; P89; Mitochondrial Contact Site And Cristae Organizing System Subunit 60; Mitochondrial Inner Membrane Organizing System 2; Cell Proliferation-Inducing Gene 4/52 Protein; Mitochondrial Inner Membrane Protein; MICOS Complex Subunit MIC60; Heart Muscle Protein; P87/89; Inner Membrane Protein, Mitochondrial (Mitofilin); Cell Proliferation-Inducing Protein 52; Proliferation-Inducing Gene 4; Motor Protein; PIG52; MIC60; PIG4
Background	Gene Name: IMMT NCBI Gene Entry: 10989 UniProt Entry: Q16891
Application Information	Molecular Weight: Predicted, 84 kDa; observed, 75 kDa Clonality: Mouse monoclonal antibody Clone ID: 24GB14355 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	Recombinant protein of human IMMT
Isotype	Mouse IgG1
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:400-1:2,000 Flow Cytometry (FCM): 1:1,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of IMMT expression in HepG2 cells using anti-IMMT antibody (Cat#65108, 1:1,000) . Green, isotype control; red, IMMT.
Immunocytochemical staining of HepG2 cells with anti-IMMT antibody (Cat#65108, 1:1,000) . Nuclei were stained blue with DAPI; IMMT was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-IMMT antibody (Cat#65108). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-IMMT antibody (Cat#65108, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-IMMT antibody (Cat#65108). IMMT expression in wild-type (WT) and IMMT shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-IMMT antibody (Cat#65108, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

IMMT
货号：

65108
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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