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KD-Validated Anti-CHMP5 Mouse Monoclonal Antibody #63819

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Flow cytometric analysis of Charged multivesicular body protein 5 expression in HepG2 cells using anti-Charged multivesicular body protein 5 antibody (Cat#63819, 1:1,000). Green, isotype control; red, Charged multivesicular body protein 5.
Western blotting analysis using anti-charged multivesicular body protein 5 antibody (Cat#63819). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-charged multivesicular body protein 5 antibody (Cat#63819, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-charged multivesicular body protein 5 antibody (Cat#63819). Charged multivesicular body protein 5 expression in wild-type (WT) and charged multivesicular body protein 5 (CHMP5) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-charged multivesicular body protein 5 antibody (Cat#63819, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    CHMP5
  • 货号:
    63819
  • 应用:
    WB, FCM
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KD-Validated Anti-CHMP5 Mouse Monoclonal Antibody
Aliases CHMP5; Charged Multivesicular Body Protein 5; Vps60; HSPC177; C9orf83; SNF7DC2; CGI-34; Vacuolar Protein Sorting-Associated Protein 60; SNF7 Domain-Containing Protein; Chromatin-Modifying Protein 5; HVps60; Chromosome 9 Open Reading Frame 83; Apoptosis-Related Protein PNAS-2; Chromatin Modifying Protein 5; SNF7 Domain Containing; PNAS-2
Background

Gene Name: CHMP5

NCBI Gene Entry: 51510

UniProt Entry: Q9NZZ3

Application Information

Molecular Weight: Predicted, 25 kDa; observed, 30 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB8030

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen Recombinant protein of human CHMP5
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:200-1:1,000
Flow Cytometry (FCM): 1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Charged multivesicular body protein 5 expression in HepG2 cells using anti-Charged multivesicular body protein 5 antibody (Cat#63819, 1:1,000). Green, isotype control; red, Charged multivesicular body protein 5.
  • Western blotting analysis using anti-charged multivesicular body protein 5 antibody (Cat#63819). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-charged multivesicular body protein 5 antibody (Cat#63819, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-charged multivesicular body protein 5 antibody (Cat#63819). Charged multivesicular body protein 5 expression in wild-type (WT) and charged multivesicular body protein 5 (CHMP5) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-charged multivesicular body protein 5 antibody (Cat#63819, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of Charged multivesicular body protein 5 expression in HepG2 cells using anti-Charged multivesicular body protein 5 antibody (Cat#63819, 1:1,000). Green, isotype control; red, Charged multivesicular body protein 5.
Western blotting analysis using anti-charged multivesicular body protein 5 antibody (Cat#63819). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-charged multivesicular body protein 5 antibody (Cat#63819, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-charged multivesicular body protein 5 antibody (Cat#63819). Charged multivesicular body protein 5 expression in wild-type (WT) and charged multivesicular body protein 5 (CHMP5) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-charged multivesicular body protein 5 antibody (Cat#63819, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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