Flow cytometric analysis of Charged multivesicular body protein 5 expression in HepG2 cells using anti-Charged multivesicular body protein 5 antibody (Cat#63819, 1:1,000). Green, isotype control; red, Charged multivesicular body protein 5.
Western blotting analysis using anti-charged multivesicular body protein 5 antibody (Cat#63819). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-charged multivesicular body protein 5 antibody (Cat#63819, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-charged multivesicular body protein 5 antibody (Cat#63819). Charged multivesicular body protein 5 expression in wild-type (WT) and charged multivesicular body protein 5 (CHMP5) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-charged multivesicular body protein 5 antibody (Cat#63819, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).