Flow cytometric analysis of Epithelial cell transforming 2 expression in HT-1080 cells using anti-Epithelial cell transforming 2 antibody (Cat#63867, 1:1,000). Green, isotype control; red, Epithelial cell transforming 2.
Immunocytochemical staining of HepG2 cells with anti-Epithelial cell transforming 2 antibody (Cat#63867, 1:500) . Nuclei were stained blue with DAPI; Epithelial cell transforming 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-epithelial cell transforming 2 antibody (Cat#63867). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-epithelial cell transforming 2 antibody (Cat#63867, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-epithelial cell transforming 2 antibody (Cat#63867). Epithelial cell transforming 2 expression in wild type (WT) and epithelial cell transforming 2 (ECT2) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-epithelial cell transforming 2 antibody (Cat#63867, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HeLa cells using anti-Epithelial cell transforming 2 antibody (Cat#63867, 1:1,000), Top panel: wild-type (WT); Bottom panal: Epithelial cell transforming 2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Epithelial cell transforming 2 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
