Flow cytometric analysis of PI3 Kinase p110 beta expression in HT-1080 cells using PI3 Kinase p110 beta antibody (Cat#64311, 1:2,000). Green, isotype control; red, PI3 Kinase p110 beta.
Immunocytochemical staining of HepG2 cells with anti-PI3 Kinase p110 beta antibody (Cat#64311, 1:1,000) . Nuclei were stained blue with DAPI; PI3 Kinase p110 beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-PI3 Kinase p110 beta antibody (Cat#64311). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PI3 Kinase p110 beta antibody (Cat#64311, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-PI3 Kinase p110 beta antibody (Cat#64311). PI3 Kinase p110 beta expression in wild type (WT) and PI3 Kinase p110 beta shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-PI3 Kinase p110 beta antibody (Cat#64311, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of PI3 Kinase p110 beta knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HT-1080 cells were stained with anti-PI3 Kinase p110 beta antibody (Cat#64311, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HT-1080 cells using anti-PI3 Kinase p110 beta antibody (Cat#64311, 1:1,000), Top panel: wild-type (WT); Bottom panal: PI3 Kinase p110 beta shRNA knockdown (KD). Nuclei were stained blue with DAPI; PI3 Kinase p110 beta was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
