Flow cytometric analysis of WDR77 expression in HeLa cells using anti-WDR77 antibody (Cat#64870, 1:2,000). Green, isotype control; red, WDR77.
Immunocytochemical staining of Hela cells with anti-WDR77 associated protein antibody (Cat#64870, 1:1,000) . Nuclei were stained blue with DAPI; WDR77 associated protein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-WDR77 antibody (Cat#64870). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-WDR77 antibody (Cat#64870, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-WDR77 antibody (Cat#64870). WDR77 expression in wild-type (WT) and WDR77 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-WDR77 antibody (Cat#64870, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of WDR77 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-WDR77 antibody (Cat#64870, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-WDR77 antibody (Cat#64870, 1:1,000), Top panel: wild-type (WT); Bottom panal: WDR77 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Acyl-CoA dehydrogenase short chain was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
