KO-Validated Anti-SMAC Rabbit Monoclonal Antibody - 产品中心

KO-Validated Anti-SMAC Rabbit Monoclonal Antibody #71175

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Flow cytometric analysis of SMAC expression in HepG2 cells using SMAC antibody (Cat#71175, 1:2,000). Green, isotype control; red, SMAC.

Immunocytochemical staining of HepG2 cells with anti-SMAC antibody (Cat#71175, 1:1,000). Nuclei were stained blue with DAPI; SMAC was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using anti-DIABLO antibody (Cat#71175). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DIABLO antibody (Cat#71175, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Western blotting analysis using anti-SMAC antibody (Cat#71175). SMAC expression in wild-type (WT) and DIABLO knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-SMAC antibody (Cat#71175, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

DIABLO
货号：

71175
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

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Information

Product Name	KO-Validated Anti-SMAC Rabbit Monoclonal Antibody
Aliases	Diablo; IAP-Binding Mitochondrial Protein; DFNA64; Second Mitochondria-Derived Activator Of Caspase; Diablo Homolog; Mitochondrial; SMAC; Smac; Direct IAP-Binding Protein With Low PI
Background	Gene Name: DIABLO NCBI Gene Entry: 56616 UniProt Entry: Q9NR28
Application Information	Molecular Weight: Predicted, 27 kDa; observed, 21 kDa Clonality: Rabbit monoclonal antibody Clone ID: 25GB2055 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Smac/Diablo
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of SMAC expression in HepG2 cells using SMAC antibody (Cat#71175, 1:2,000). Green, isotype control; red, SMAC.
Immunocytochemical staining of HepG2 cells with anti-SMAC antibody (Cat#71175, 1:1,000). Nuclei were stained blue with DAPI; SMAC was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-DIABLO antibody (Cat#71175). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DIABLO antibody (Cat#71175, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-SMAC antibody (Cat#71175). SMAC expression in wild-type (WT) and DIABLO knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-SMAC antibody (Cat#71175, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

DIABLO
货号：

71175
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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