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KD-Validated Anti-NTH1 Rabbit Monoclonal Antibody #64908

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Flow cytometric analysis of NTHL1 expression in HAP-1 cells using anti-NTHL1 antibody (Cat#64908, 1:2,000). Green, isotype control; red, NTHL1.
Immunocytochemical staining of HAP-1 cells with anti-NTHL1 antibody (Cat#64908, 1:1,000) . Nuclei were stained blue with DAPI; NTHL1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Low . Scale bar, 20 μm.
Western blotting analysis using anti-NTHL1 antibody (Cat#64908). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NTHL1 antibody (Cat#64908, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-NTHL1 antibody (Cat#64908). NTHL1 expression in wild-type (WT) and NTHL1 knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NTHL1 antibody (Cat#64908, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名：
    NTHL1
  • 货号：
    64908
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-NTH1 Rabbit Monoclonal Antibody
Aliases NTHL1; Nth Like DNA Glycosylase 1; OCTS3; NTH1; Bifunctional DNA N-Glycosylase/DNA-(Apurinic Or Apyrimidinic Site) Lyase; Endonuclease III-Like Protein 1; DNA Glycosylase/AP Lyase; HNTH1; Bifunctional DNA N-Glycoslyase/DNA-(Apurinic Or Apyrimidinic Site) Lyase; Nth Endonuclease III-Like 1 (E. Coli); Nth (E.Coli Endonuclease III)-Like 1; Nth Endonuclease III-Like 1; DNA Glycoslyase/AP Lyase; EC 4.2.99.18; EC 3.2.2.-;FAP3
Background

Gene Name: NTHL1

NCBI Gene Entry: 4913

UniProt Entry: P78549
Application Information

Molecular Weight: Predicted, 34 kDa; observed, 34 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB13700

Species Reactivity: Human, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen Recombinant protein of human NTH1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of NTHL1 expression in HAP-1 cells using anti-NTHL1 antibody (Cat#64908, 1:2,000). Green, isotype control; red, NTHL1.
  • Immunocytochemical staining of HAP-1 cells with anti-NTHL1 antibody (Cat#64908, 1:1,000) . Nuclei were stained blue with DAPI; NTHL1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Low . Scale bar, 20 μm.
  • Western blotting analysis using anti-NTHL1 antibody (Cat#64908). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NTHL1 antibody (Cat#64908, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-NTHL1 antibody (Cat#64908). NTHL1 expression in wild-type (WT) and NTHL1 knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NTHL1 antibody (Cat#64908, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
  • 基因名：
    NTHL1
  • 货号：
    64908
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of NTHL1 expression in HAP-1 cells using anti-NTHL1 antibody (Cat#64908, 1:2,000). Green, isotype control; red, NTHL1.
Immunocytochemical staining of HAP-1 cells with anti-NTHL1 antibody (Cat#64908, 1:1,000) . Nuclei were stained blue with DAPI; NTHL1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Low . Scale bar, 20 μm.
Western blotting analysis using anti-NTHL1 antibody (Cat#64908). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NTHL1 antibody (Cat#64908, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-NTHL1 antibody (Cat#64908). NTHL1 expression in wild-type (WT) and NTHL1 knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NTHL1 antibody (Cat#64908, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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