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KD-Validated Anti-SRC Rabbit Polyclonal Antibody #64991

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Western blotting analysis using anti-SRC antibody (Cat#64991). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SRC antibody (Cat#64991, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-SRC antibody (Cat #64991). SRC expression in wild-type (WT) and SRC shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-SRC antibody (Cat #64991, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat #201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat #226).
  • 基因名:
    SRC
  • 货号:
    64991
  • 应用:
    WB
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KD-Validated Anti-SRC Rabbit Polyclonal Antibody
Aliases SRC; SRC Proto-Oncogene, Non-Receptor Tyrosine Kinase; C-Src; SRC1; ASV; V-Src Avian Sarcoma (Schmidt-Ruppin A-2) Viral Oncogene Homolog; Proto-Oncogene Tyrosine-Protein Kinase Src; Proto-Oncogene C-Src; EC 2.7.10.2; P60-Src; Protooncogene SRC, Rous Sarcoma; Tyrosine-Protein Kinase SRC-1; Tyrosine Kinase Pp60c-Src; Pp60c-Src; EC 2.7.10; THC6
Background

Gene Name: SRC

NCBI Gene Entry: 6714

UniProt Entry: P12931

Application Information

Molecular Weight: Predicted, 60 kDa; observed, 58 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB)

Immunogen A synthesized peptide derived from human c-SRC
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-SRC antibody (Cat#64991). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SRC antibody (Cat#64991, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-SRC antibody (Cat #64991). SRC expression in wild-type (WT) and SRC shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-SRC antibody (Cat #64991, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat #201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat #226).
Citation(0)
Western blotting analysis using anti-SRC antibody (Cat#64991). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SRC antibody (Cat#64991, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-SRC antibody (Cat #64991). SRC expression in wild-type (WT) and SRC shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-SRC antibody (Cat #64991, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat #201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat #226).
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