Western blotting analysis using anti-PIP4K2A antibody (Cat#65034). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PIP4K2A antibody (Cat#65034, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-PIP4K2A antibody (Cat#65034). PIP4K2A expression in wild-type (WT) and PIP4K2A shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PIP4K2A antibody (Cat#65034, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of PIP4K2A expression in HT-1080 cells using anti-PIP4K2A antibody (Cat#65034, 1:2,000). Green, isotype control; red, PIP4K2A.
Flow cytometric analysis of PIP4K2A expression in HT-1080 cells using anti-PIP4K2A antibody (Cat#65034, 1:2,000). Green, isotype control; red, PIP4K2A.