Western blotting analysis using anti-retinol dehydrogenase 14 antibody (Cat#65035). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-retinol dehydrogenase 14 antibody (Cat#65035, 1:500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-retinol dehydrogenase 14 antibody (Cat#65035). Retinol dehydrogenase 14 expression in wild-type (WT) and retinol dehydrogenase 14 (RDH14) knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-retinol dehydrogenase 14 antibody (Cat#65035, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
RDH14; Retinol Dehydrogenase 14; SDR7C4; PAN2; Short Chain Dehydrogenase/Reductase Family 7C Member 4; Retinol Dehydrogenase 14 (All-Trans/9-Cis/11-Cis); Retinol Dehydrogenase 14 (All-Trans And 9-Cis); Alcohol Dehydrogenase PAN2; Short Chain Dehydrogenase/Reductase Family 7C, Member 4; Pancreas Protein 2; EC 1.1.1.300
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:100-1:500
Note
This product is for research use only.
Picture
Western blotting analysis using anti-retinol dehydrogenase 14 antibody (Cat#65035). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-retinol dehydrogenase 14 antibody (Cat#65035, 1:500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-retinol dehydrogenase 14 antibody (Cat#65035). Retinol dehydrogenase 14 expression in wild-type (WT) and retinol dehydrogenase 14 (RDH14) knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-retinol dehydrogenase 14 antibody (Cat#65035, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-retinol dehydrogenase 14 antibody (Cat#65035). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-retinol dehydrogenase 14 antibody (Cat#65035, 1:500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-retinol dehydrogenase 14 antibody (Cat#65035). Retinol dehydrogenase 14 expression in wild-type (WT) and retinol dehydrogenase 14 (RDH14) knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-retinol dehydrogenase 14 antibody (Cat#65035, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
