KD-Validated Anti-Cyclin E1 Rabbit Monoclonal Antibody - 产品中心

KD-Validated Anti-Cyclin E1 Rabbit Monoclonal Antibody #65259

一键复制产品信息

返回列表

Western blotting analysis using anti-cyclin E1 antibody (Cat#65259). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cyclin E1 antibody (Cat#65259, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using anti-cyclin E1 antibody (Cat#65259). Cyclin E1 expression in wild-type (WT) and cyclin E1 (CCNE1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-cyclin E1 antibody (Cat#65259, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Flow cytometric analysis of CCNE1 expression in HeLa cells using anti-CCNE1 antibody (Cat#65259, 1:2,000). Green, isotype control; red, CCNE1.

基因名：

CCNE1
货号：

65259
应用：

WB, FCM
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information

Product Name	KD-Validated Anti-Cyclin E1 Rabbit Monoclonal Antibody
Aliases	CCNE1; Cyclin E1; CCNE; G1/S-Specific Cyclin-E1; Cyclin Es; Cyclin Et; PCCNE1
Background	Gene Name: CCNE1 NCBI Gene Entry: 898 UniProt Entry: P24864
Application Information	Molecular Weight: Predicted, 48 kDa; observed, 48 kDa Clonality: Rabbit monoclonal antibody Clone ID: 25GB400 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human Cyclin E1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000
Note	This product is for research use only.

Picture

Western blotting analysis using anti-cyclin E1 antibody (Cat#65259). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cyclin E1 antibody (Cat#65259, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-cyclin E1 antibody (Cat#65259). Cyclin E1 expression in wild-type (WT) and cyclin E1 (CCNE1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-cyclin E1 antibody (Cat#65259, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of CCNE1 expression in HeLa cells using anti-CCNE1 antibody (Cat#65259, 1:2,000). Green, isotype control; red, CCNE1.

Citation(0)

基因名：

CCNE1
货号：

65259
应用：

WB, FCM
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

首页

关于我们

善本标准

产品中心

定制服务

信号通路

促销活动

技术支持

新闻资讯

首页