Flow cytometric analysis of Ezrin expression in HAP-1 cells using Ezrin antibody (Cat#71193, 1:2,000). Green, isotype control; red, Ezrin.
Immunocytochemical staining of HAP-1 cells with anti-Ezrin antibody (Cat#71193, 1:1,000). Nuclei were stained blue with DAPI; Ezrin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-ezrin antibody (Cat#71193). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ezrin antibody (Cat#71193, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-ezrin antibody (Cat#71193). Ezrin expression in wild-type (WT) and ezrin (EZR) knockout (KO) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-ezrin antibody (Cat#71193, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
