Flow cytometric analysis of TRIM34 expression in HaCaT cells using anti-TRIM34 antibody (Cat#65083, 1:2,000) . Green, isotype control; red, TRIM34.
Western blotting analysis using anti-TRIM34 antibody (Cat#65083). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TRIM34 antibody (Cat#65083, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-TRIM34 antibody (Cat#65083). TRIM34 expression in wild-type (WT) and TRIM34 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-TRIM34 antibody (Cat#65083, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).