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KD-Validated Anti-TPX2 Rabbit Monoclonal Antibody #65663

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Western blotting analysis using anti-TPX2 antibody (Cat#65663). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TPX2 antibody (Cat#65663, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Western blotting analysis using anti-TPX2 antibody (Cat#65663). TPX2 expression in wild-type (WT) and TPX2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-TPX2 antibody (Cat#65663, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Flow cytometric analysis of TPX2 expression in HAP-1 cells using anti-TPX2 antibody (Cat#65663, 1:2,000). Green, isotype control; red, TPX2.

基因名：

TPX2
货号：

65663
应用：

WB, FCM
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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Information

Product Name	KD-Validated Anti-TPX2 Rabbit Monoclonal Antibody
Aliases	TPX2; TPX2 Microtubule Nucleation Factor; HCA519; DIL-2; P100; Hepatocellular Carcinoma-Associated Antigen 519; Targeting Protein For Xklp2; C20orf1; C20orf2; FLS353; Differentially Expressed In Cancerous And Non-Cancerous Lung Cells 2; Differentially Expressed In Cancerous And Noncancerous Lung Cells 2; Hepatocellular Carcinoma-Associated Antigen 90; Protein Fls353; DIL2; Restricted Expression Proliferation Associated Protein 100; Restricted Expression Proliferation-Associated Protein 100; TPX2, Microtubule-Associated, Homolog (Xenopus Laevis); Preferentially Expressed In Colorectal Cancer; TPX2, Microtubule-Associated Protein Homolog; Differentially Expressed In Lung Cells; TPX2, Microtubule-Associated, Homolog; Chromosome 20 Open Reading Frame 1; TPX2, Microtubule-Associated; GD:C20orf1; REPP86; HCTP4
Background	Gene Name: TPX2 NCBI Gene Entry: 22974 UniProt Entry: Q9ULW0
Application Information	Molecular Weight: Predicted, 86 kDa; observed, 98 kDa Clonality: Rabbit monoclonal antibody Clone ID: 25GB4765 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human TPX2
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000
Note	This product is for research use only.

Picture

Western blotting analysis using anti-TPX2 antibody (Cat#65663). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TPX2 antibody (Cat#65663, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-TPX2 antibody (Cat#65663). TPX2 expression in wild-type (WT) and TPX2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-TPX2 antibody (Cat#65663, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of TPX2 expression in HAP-1 cells using anti-TPX2 antibody (Cat#65663, 1:2,000). Green, isotype control; red, TPX2.

Citation(0)

基因名：

TPX2
货号：

65663
应用：

WB, FCM
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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