Flow cytometric analysis of TTLL12 expression in HepG2 cells using anti-TTLL12 antibody (Cat#65831, 1:2,000). Green, isotype control; red, TTLL12.
Western blotting analysis using anti-TTLL12 antibody (Cat#65831). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TTLL12 antibody (Cat#65831, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-TTLL12 antibody (Cat#65831). TTLL12 expression in wild-type (WT) and TTLL12 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-TTLL12 antibody (Cat#65831, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
TTLL12; Tubulin Tyrosine Ligase Like 12; KIAA0153; Inactive Tubulin--Tyrosine Ligase-Like Protein 12; Tubulin--Tyrosine Ligase-Like Protein 12; Tubulin Tyrosine Ligase-Like Family, Member 12; Tubulin Tyrosine Ligase-Like Family Member 12; DJ526I14.2
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human TTLL12
Isotype
Mouse IgG2a
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500 Flow Cytometry (FCM): 1:2,000
Note
This product is for research use only.
Picture
Flow cytometric analysis of TTLL12 expression in HepG2 cells using anti-TTLL12 antibody (Cat#65831, 1:2,000). Green, isotype control; red, TTLL12.
Western blotting analysis using anti-TTLL12 antibody (Cat#65831). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TTLL12 antibody (Cat#65831, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-TTLL12 antibody (Cat#65831). TTLL12 expression in wild-type (WT) and TTLL12 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-TTLL12 antibody (Cat#65831, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of TTLL12 expression in HepG2 cells using anti-TTLL12 antibody (Cat#65831, 1:2,000). Green, isotype control; red, TTLL12.
Western blotting analysis using anti-TTLL12 antibody (Cat#65831). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TTLL12 antibody (Cat#65831, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-TTLL12 antibody (Cat#65831). TTLL12 expression in wild-type (WT) and TTLL12 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-TTLL12 antibody (Cat#65831, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
