Flow cytometric analysis of Flotillin 1 expression in HepG2 cells using anti-Flotillin 1 antibody (Cat#67451,1:2,000). Green, isotype control; red, Flotillin 1.
Immunocytochemical staining of HepG2 cells with Flotillin 1 antibody (Cat#67451,1:1,000). Nuclei were stained blue with DAPI; Flotillin 1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Western blotting analysis using anti-Flotillin 1 antibody (Cat#67451). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Flotillin 1 antibody (Cat#67451,1:10,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201,1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Flotillin 1 antibody (Cat#67451). Flotillin 1 expression in wild type (WT) and Flotillin 1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Flotillin 1 antibody (Cat#67451,1:10,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201,1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of Flotillin 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Flotillin 1 antibody (Cat#67451, 1:2,000) and analyzed using CytoFLEX.
Immunocytochemical staining of HeLa cells using anti-Flotillin 1 antibody (Cat#67451, 1:1,000), Top panel: wild-type (WT); Bottom panal: Flotillin 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Flotillin 1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.