KD-Validated Anti-Smad4 Rabbit Monoclonal Antibody - 产品中心

KD-Validated Anti-Smad4 Rabbit Monoclonal Antibody #67514

一键复制产品信息

返回列表

Flow cytometric analysis of Smad4 expression in C2C12 cells using Smad4 antibody (Cat#67514,1:2,000). Green, isotype control; red, Smad4.

Immunocytochemical staining of C2C12 cells with Smad4 antibody (Cat#67514, 1:1,000). Nuclei were stained blue with DAPI; Smad4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-Smad4 antibody (Cat#67514). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Smad4 antibody (Cat#67514,1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201,1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using anti-Smad4 antibody (Cat#67514). Smad4 expression in wild type (WT) and Smad4 shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Smad4 antibody (Cat#67514,1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201,1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

SMAD4
货号：

67514
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information

Product Name	KD-Validated Anti-Smad4 Rabbit Monoclonal Antibody
Aliases	DPC4; MADH4; Mothers Against Decapentaplegic Homolog 4; Deletion Target In Pancreatic Carcinoma 4; MAD Homolog 4; MAD, Mothers Against Decapentaplegic Homolog 4 (Drosophila); Mothers Against Decapentaplegic, Drosophila, Homolog Of, 4; SMAD, Mothers Against DPP Homolog 4 (Drosophila); Deleted In Pancreatic Carcinoma Locus 4; SMAD, Mothers Against DPP Homolog 4; Mothers Against DPP Homolog 4; SMAD 4; HSMAD4; MYHRS; Smad4; JIP
Background	Gene Name: SMAD4 NCBI Gene Entry: 4089 UniProt Entry: Q13485
Application Information	Molecular Weight: Predicted, 60 kDa, observed, 65 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB975 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Smad4
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Smad4 expression in C2C12 cells using Smad4 antibody (Cat#67514,1:2,000). Green, isotype control; red, Smad4.
Immunocytochemical staining of C2C12 cells with Smad4 antibody (Cat#67514, 1:1,000). Nuclei were stained blue with DAPI; Smad4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Smad4 antibody (Cat#67514). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Smad4 antibody (Cat#67514,1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201,1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Smad4 antibody (Cat#67514). Smad4 expression in wild type (WT) and Smad4 shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Smad4 antibody (Cat#67514,1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201,1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

SMAD4
货号：

67514
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

首页

关于我们

善本标准

产品中心

定制服务

信号通路

促销活动

技术支持

新闻资讯

首页