Flow cytometric analysis of Bcl-2 expression in HepG2 cells using anti-Bcl-2 antibody (Cat#68142, 1:2,000). Green, isotype control;red, Bcl-2.
Immunocytochemical staining of HepG2 cells with Bcl-2 antibody (Cat#68142, 1:1,000). Nuclei were stained blue with DAPI; Bcl-2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Bcl 2 antibody (Cat#68142). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Bcl 2 antibody (Cat#68142, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Bcl-2 antibody (Cat#68142). Bcl-2 expression in wild type (WT) and Bcl-2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Bcl-2 antibody (Cat#68142, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of Bcl-2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Bcl-2 antibody (Cat#68142, 1:2,000) and analyzed using BD flow cytometer.
