Flow cytometric analysis of Src in HAP-1 cells using Src antibody (Cat#68611, 1:2,000). Green, isotype control; red, Src.
Immunocytochemical staining of HAP-1 cells with Src antibody (Cat#68611, 1:1,000). Nuclei were stained blue with DAPI; Src was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Src antibody (Cat#68611). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Src antibody (Cat#68611, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Src antibody (Cat#68611). Src expression in wild type (WT) and Src shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Src antibody (Cat#68611, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of Src knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HT1080 cells were stained with anti-Src antibody (Cat#68611, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HT1080 cells using anti-Src antibody (Cat#68611, 1:1,000), Top panel: wild-type (WT); Bottom panal: Src shRNA knockdown (KD). Nuclei were stained blue with DAPI; Src was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
