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KO-Validated Anti-ELOVL5 Rabbit Monoclonal Antibody #71184

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Flow cytometric analysis of ELOVL5 expression in HepG2 cells using ELOVL5 antibody (Cat#71184, 1:2,000). Green, isotype control; red, ELOVL5.
Western blotting analysis using anti-ELOVL5 antibody (Cat#71184). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ELOVL5 antibody (Cat#71184, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-ELOVL5 antibody (Cat#71184). ELOVL5 expression in wild-type (WT) and ELOVL5 knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ELOVL5 antibody (Cat#71184, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    ELOVL5
  • 货号:
    71184
  • 应用:
    WB, FCM
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KO-Validated Anti-ELOVL5 Rabbit Monoclonal Antibody
Aliases ELOVL5; ELOVL Fatty Acid Elongase 5; HELO1; DJ483K16.1; ELOVL Family Member 5, Elongation Of Long Chain Fatty Acids (FEN1/Elo2, SUR4/Elo3-Like, Yeast); Elongation Of Very Long Chain Fatty Acids Protein 5; Very Long Chain 3-Ketoacyl-CoA Synthase 5; Very Long Chain 3-Oxoacyl-CoA Synthase 5; 3-Keto Acyl-CoA Synthase ELOVL5; Spinocerebellar Ataxia 38; Fatty Acid Elongase 1; ELOVL FA Elongase 5; SCA38; Homolog Of Yeast Long Chain Polyunsaturated Fatty Acid Elongation Enzyme; EC 2.3.1.199; ELOVL2
Background

Gene Name: ELOVL5

NCBI Gene Entry: 60481

UniProt Entry: Q9NYP7

Application Information

Molecular Weight: Predicted, 35 kDa; observed, 26 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB2255

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human ELOVL5
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of ELOVL5 expression in HepG2 cells using ELOVL5 antibody (Cat#71184, 1:2,000). Green, isotype control; red, ELOVL5.
  • Western blotting analysis using anti-ELOVL5 antibody (Cat#71184). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ELOVL5 antibody (Cat#71184, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-ELOVL5 antibody (Cat#71184). ELOVL5 expression in wild-type (WT) and ELOVL5 knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ELOVL5 antibody (Cat#71184, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of ELOVL5 expression in HepG2 cells using ELOVL5 antibody (Cat#71184, 1:2,000). Green, isotype control; red, ELOVL5.
Western blotting analysis using anti-ELOVL5 antibody (Cat#71184). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ELOVL5 antibody (Cat#71184, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-ELOVL5 antibody (Cat#71184). ELOVL5 expression in wild-type (WT) and ELOVL5 knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ELOVL5 antibody (Cat#71184, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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