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KD-Validated Anti-Cathepsin B Rabbit Monoclonal Antibody #69771

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Immunocytochemical staining of HepG2 cells with Cathepsin B antibody (Cat#69771, 1:1,000). Nuclei were stained blue with DAPI; Cathepsin B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
Western blotting analysis using anti-Cathepsin B antibody (Cat#69771). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Cathepsin B antibody (Cat#69771, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Cathepsin B antibody (Cat#69771). Cathepsin B expression in wild type (WT) and Cathepsin B shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Cathepsin B antibody (Cat#69771, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    CTSB
  • 货号:
    69771
  • 应用:
    WB, IC
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KD-Validated Anti-Cathepsin B Rabbit Monoclonal Antibody
Aliases CTSB; Cathepsin B; APP Secretase; Cathepsin B1; EC 3.4.22.1; APPS; CPSB; Keratolytic Winter Erythema (Oudtshoorn Skin Disease); Epididymis Secretory Sperm Binding Protein; Amyloid Precursor Protein Secretase; Cysteine Protease; EC 3.4.22; RECEUP; KWE
Background

Gene Name: CTSB

NCBI Gene Entry: 1508

UniProt Entry: P07858

Application Information

Molecular Weight: Predicted, 38 kDa, observed, 38 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB1130

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human Cathepsin B
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Immunocytochemical staining of HepG2 cells with Cathepsin B antibody (Cat#69771, 1:1,000). Nuclei were stained blue with DAPI; Cathepsin B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
  • Western blotting analysis using anti-Cathepsin B antibody (Cat#69771). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Cathepsin B antibody (Cat#69771, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-Cathepsin B antibody (Cat#69771). Cathepsin B expression in wild type (WT) and Cathepsin B shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Cathepsin B antibody (Cat#69771, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Immunocytochemical staining of HepG2 cells with Cathepsin B antibody (Cat#69771, 1:1,000). Nuclei were stained blue with DAPI; Cathepsin B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
Western blotting analysis using anti-Cathepsin B antibody (Cat#69771). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Cathepsin B antibody (Cat#69771, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Cathepsin B antibody (Cat#69771). Cathepsin B expression in wild type (WT) and Cathepsin B shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Cathepsin B antibody (Cat#69771, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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