Anti-Raf1 Rabbit Monoclonal Antibody - 产品中心 - 合肥善本生物科技有限公司

Anti-Raf1 Rabbit Monoclonal Antibody #4260

一键复制产品信息

返回列表

Flow cytometric analysis of Raf1 expression in HeLa cells using anti-Raf1 antibody (Cat#4260, 1:2,000). Green, isotype control; red, Raf1.

Immunocytochemical staining of HeLa cells with anti-RAF1 antibody(Cat#4260, 1:1,000). Nuclei were stained blue with DAPI;
RAF1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-Raf1 antibody (Cat#4260). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Raf1 antibody (Cat#4260, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

RAF1
货号：

4260
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-Raf1 Rabbit Monoclonal Antibody
Aliases	RAF1; Raf-1 Proto-Oncogene, Serine/Threonine Kinase; Raf-1; CRAF; RAF Proto-Oncogene Serine/Threonine-Protein Kinase; V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1; C-Raf Proto-Oncogene, Serine/Threonine Kinase; Proto-Oncogene C-RAF; EC 2.7.11.1; C-Raf; V-Raf-1 Murine Leukemia Viral Oncogene-Like Protein 1; Raf Proto-Oncogene Serine/Threonine Protein Kinase; Oncogene RAF1; EC 2.7.11; CMD1NN; C-RAF; RAF-1; CRaf; NS5; RAF
Background	Gene Name: RAF1 NCBI Gene Entry: 5894 UniProt Entry: P04049
Application Information	Molecular Weight: Predicted, 73 kDa; observed, 73 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB10330 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Phospho-Raf1 (S621)
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Raf1 expression in HeLa cells using anti-Raf1 antibody (Cat#4260, 1:2,000). Green, isotype control; red, Raf1.
Immunocytochemical staining of HeLa cells with anti-RAF1 antibody(Cat#4260, 1:1,000). Nuclei were stained blue with DAPI;
RAF1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-Raf1 antibody (Cat#4260). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Raf1 antibody (Cat#4260, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

RAF1
货号：

4260
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

首页

关于我们

善本标准

产品中心

定制服务

信号通路

促销活动

技术支持

新闻资讯

首页