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KO-Validated Anti-HLA-C Mouse Monoclonal Antibody #71122

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Western blotting analysis using anti-HLA-C antibody (Cat#71122). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HLA-C antibody (Cat#71122, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Western blotting analysis using anti-HLA-C antibody (Cat#71122). HLA-C expression in wild type (WT) and HLA-C knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-HLA-C antibody (Cat#71122, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Flow cytometric analysis of HLA-C expression in HAP-1 cells using anti-HLA-C antibody (Cat#71122, 1:2,000). Green, isotype control; red, HLA-C.

基因名：

HLA-C
货号：

71122
应用：

WB, FCM
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

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Information

Product Name	KO-Validated Anti-HLA-C Mouse Monoclonal Antibody
Aliases	HLA-C; Major Histocompatibility Complex, Class I, C; HLA Class I Histocompatibility Antigen, C Alpha Chain; HLA-JY3; D6S204; PSORS1; HLAC; Major Histocompatibility Antigen HLA-C; MHC Class I Antigen Heavy Chain HLA-C; Human Leukocyte Antigen-C Alpha Chain; Psoriasis Susceptibility 1; Human Leukocyte Antigen C; HLA-C Antigen; HLA-Cw; HLC-C; MHC
Background	Gene Name: HLA-C NCBI Gene Entry: 107 UniProt Entry: P10321
Application Information	Molecular Weight: Predicted, 41 kDa; Observed, 40 kDa Clonality: Mouse monoclonal antibody Clone ID: 24GB8545 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	Recombinant protein of human HLA-C
Isotype	Mouse IgG
Storage Buffer	Supplied in TBS (pH 7.4) containing 50% glycerol, 1% BSA and 0.02% Proclin 300.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000
Note	This product is for research use only.

Picture

Western blotting analysis using anti-HLA-C antibody (Cat#71122). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HLA-C antibody (Cat#71122, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-HLA-C antibody (Cat#71122). HLA-C expression in wild type (WT) and HLA-C knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-HLA-C antibody (Cat#71122, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of HLA-C expression in HAP-1 cells using anti-HLA-C antibody (Cat#71122, 1:2,000). Green, isotype control; red, HLA-C.

Citation(0)

基因名：

HLA-C
货号：

71122
应用：

WB, FCM
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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