Flow cytometric analysis of alpha Actinin 4 expression in HepG2 cells using anti-alpha Actinin 4 antibody (Cat# 71140, 1:2,000). Green, isotype control; red, alpha Actinin 4.
Western blotting analysis using anti-alpha Actinin 4 antibody (Cat#71140). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-alpha Actinin 4 antibody (Cat#71140, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-alpha actinin 4 antibody (Cat#71140). Alpha actinin 4 expression in wild type (WT) and alpha actinin 4 (ACTN4) knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-alpha actinin 4 antibody (Cat#71140, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
A synthesized peptide derived from human alpha Actinin 4
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000
Note
This product is for research use only.
Picture
Flow cytometric analysis of alpha Actinin 4 expression in HepG2 cells using anti-alpha Actinin 4 antibody (Cat# 71140, 1:2,000). Green, isotype control; red, alpha Actinin 4.
Western blotting analysis using anti-alpha Actinin 4 antibody (Cat#71140). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-alpha Actinin 4 antibody (Cat#71140, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-alpha actinin 4 antibody (Cat#71140). Alpha actinin 4 expression in wild type (WT) and alpha actinin 4 (ACTN4) knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-alpha actinin 4 antibody (Cat#71140, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of alpha Actinin 4 expression in HepG2 cells using anti-alpha Actinin 4 antibody (Cat# 71140, 1:2,000). Green, isotype control; red, alpha Actinin 4.
Western blotting analysis using anti-alpha Actinin 4 antibody (Cat#71140). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-alpha Actinin 4 antibody (Cat#71140, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-alpha actinin 4 antibody (Cat#71140). Alpha actinin 4 expression in wild type (WT) and alpha actinin 4 (ACTN4) knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-alpha actinin 4 antibody (Cat#71140, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
