Flow cytometric analysis of Cyclin dependent kinase inhibitor 2A expression in HepG2 cells using Cyclin dependent kinase inhibitor 2A antibody (Cat#71177, 1:2,000). Green, isotype control; red, Cyclin dependent kinase inhibitor 2A.
Immunocytochemical staining of HepG2 cells with anti-Cyclin dependent kinase inhibitor 2A antibody (Cat#71177, 1:1,000). Nuclei were stained blue with DAPI; Cyclin dependent kinase inhibitor 2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-cyclin dependent kinase inhibitor 2A antibody (Cat#71177). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cyclin dependent kinase inhibitor 2A antibody (Cat#71177, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-cyclin dependent kinase inhibitor 2A antibody (Cat#71177). Cyclin dependent kinase inhibitor 2A expression in wild-type (WT) and cyclin dependent kinase inhibitor 2A (CDKN2A) knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-cyclin dependent kinase inhibitor 2A antibody (Cat#71177, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
