Flow cytometric analysis of AKT1 expression in C2C12 cells using anti-AKT1 antibody (Cat#3449, 1:2,000). Green, isotype control; red, AKT1.
Immunocytochemical staining of C2C12 cells with anti-AKT1 antibody (Cat#3449, 1:1,000). Nuclei were stained blue with DAPI; AKT1 was stained magenta with Alexa Fluor庐 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 渭m.
Western blotting analysis using anti-AKT1 antibody (Cat#3449). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-AKT1 antibody (Cat#3449, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of AKT1 expression in C2C12 cells using anti-AKT1 antibody (Cat#3449, 1:2,000). Green, isotype control; red, AKT1.
Immunocytochemical staining of C2C12 cells with anti-AKT1 antibody (Cat#3449, 1:1,000). Nuclei were stained blue with DAPI; AKT1 was stained magenta with Alexa Fluor庐 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 渭m.
Western blotting analysis using anti-AKT1 antibody (Cat#3449). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-AKT1 antibody (Cat#3449, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of AKT1 expression in C2C12 cells using anti-AKT1 antibody (Cat#3449, 1:2,000). Green, isotype control; red, AKT1.
Immunocytochemical staining of C2C12 cells with anti-AKT1 antibody (Cat#3449, 1:1,000). Nuclei were stained blue with DAPI; AKT1 was stained magenta with Alexa Fluor庐 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 渭m.
Western blotting analysis using anti-AKT1 antibody (Cat#3449). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-AKT1 antibody (Cat#3449, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
