Flow cytometric analysis of Integrin subunit alpha 4 expression in HAP-1 cells using Integrin subunit alpha 4 antibody (Cat#71181, 1:2,000). Green, isotype control; red, Integrin subunit alpha 4.
Immunocytochemical staining of HAP-1 cells with anti-Integrin subunit alpha 4 antibody (Cat#71181, 1:1,000). Nuclei were stained blue with DAPI; Integrin subunit alpha 4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-integrin α4 antibody (Cat#71181). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-integrin α4 antibody (Cat#71181, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-integrin subunit alpha 4 antibody (Cat#71181). Integrin subunit alpha 4 expression in wild-type (WT) and integrin subunit alpha 4 (ITGA4) knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-integrin subunit alpha 4 antibody (Cat#71181, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
