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Anti-NDUFB8 Rabbit Monoclonal Antibody #3012

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Flow cytometric analysis of NDUFB8 expression in HepG2 cells using anti-NDUFB8 antibody (Cat#3012, 1:2,000). Green, isotype control; red, NDUFB8.

Immunocytochemical staining of HepG2 cells with anti-NDUFB8 antibody (Cat#3012, 1:1,000) . Nuclei were stained blue with DAPI; NDUFB8 was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-NDUFB8 antibody (Cat#3012). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NDUFB8 antibody (Cat#3012, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

NDUFB8
货号：

3012
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

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Information

Product Name	Anti-NDUFB8 Rabbit Monoclonal Antibody
Aliases	NDUFB8; NADH:Ubiquinone Oxidoreductase Subunit B8; CI-ASHI; ASHI; NADH Dehydrogenase [Ubiquinone] 1 Beta Subcomplex Subunit 8, Mitochondrial; NADH Dehydrogenase (Ubiquinone) 1 Beta Subcomplex, 8, 19kDa; Complex I ASHI Subunit; Complex I-ASHI; NADH Dehydrogenase (Ubiquinone) 1 Beta Subcomplex, 8 (19kD, ASHI); NADH:Ubiquinone Oxidoreductase ASHI Subunit; NADH-Ubiquinone Oxidoreductase ASHI Subunit; MC1DN32
Background	Gene Name: NDUFB8 NCBI Gene Entry: 4714 UniProt Entry: O95169
Application Information	Molecular Weight: Predicted, 22 kDa; observed, 19 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB3625 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human NDUFB8
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of NDUFB8 expression in HepG2 cells using anti-NDUFB8 antibody (Cat#3012, 1:2,000). Green, isotype control; red, NDUFB8.
Immunocytochemical staining of HepG2 cells with anti-NDUFB8 antibody (Cat#3012, 1:1,000) . Nuclei were stained blue with DAPI; NDUFB8 was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-NDUFB8 antibody (Cat#3012). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NDUFB8 antibody (Cat#3012, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

NDUFB8
货号：

3012
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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