Flow cytometric analysis of Neurofilament medium chain expression in Hela cells using anti-Neurofilament medium chain antibody (Cat#71201, 1:2,000). Green, isotype control; red, Neurofilament medium chain.
Immunocytochemical staining of Hela cells with anti-Neurofilament medium chain antibody (Cat#71201, 1:1,000). Nuclei were stained blue with DAPI; Neurofilament medium chain was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-neurofilament medium chain antibody (Cat#71201). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-neurofilament medium chain antibody (Cat#71201, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-neurofilament medium chain antibody (Cat#71201). Neurofilament medium chain expression in wild-type (WT) and neurofilament medium chain (NEFM) knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-neurofilament medium chain antibody (Cat#71201, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
