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KO-Validated Anti-Apolipoprotein E Rabbit Monoclonal Antibody #71212

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Western blotting analysis using anti-apolipoprotein E antibody (Cat#71212). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-apolipoprotein E antibody (Cat#71212, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-apolipoprotein E antibody (Cat#71212). Apolipoprotein E expression in wild type (WT) and apolipoprotein E (APOE) knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-apolipoprotein E antibody (Cat#71212, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    APOE
  • 货号:
    71212
  • 应用:
    WB
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KO-Validated Anti-Apolipoprotein E Rabbit Monoclonal Antibody
Aliases APOE; Apolipoprotein E; AD2; Alzheimer Disease 2 (APOE*E4-Associated, Late Onset); Apolipoprotein E3; LDLCQ5; APO-E; ApoE4; Apo-E; LPG
Background

Gene Name: APOE

NCBI Gene Entry: 348

UniProt Entry: P02649

Application Information

Molecular Weight: Predicted, 36 kDa; observed, 35 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB2880

Species Reactivity: Human

Applications Tested: Western blotting (WB)

Immunogen A synthesized peptide derived from human Apolipoprotein E
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-apolipoprotein E antibody (Cat#71212). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-apolipoprotein E antibody (Cat#71212, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-apolipoprotein E antibody (Cat#71212). Apolipoprotein E expression in wild type (WT) and apolipoprotein E (APOE) knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-apolipoprotein E antibody (Cat#71212, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Western blotting analysis using anti-apolipoprotein E antibody (Cat#71212). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-apolipoprotein E antibody (Cat#71212, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-apolipoprotein E antibody (Cat#71212). Apolipoprotein E expression in wild type (WT) and apolipoprotein E (APOE) knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-apolipoprotein E antibody (Cat#71212, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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