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KO-Validated Anti-Phospho-YAP1 (S127) Rabbit Monoclonal Antibody #71227

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Flow cytometric analysis of Phospho-YAP1 (S127) expression in HepG2 cells using anti-Phospho-YAP1 (S127) antibody (Cat#71227, 1:2,000). Green, isotype control; red, Phospho-YAP1 (S127).
Immunocytochemical staining of HepG2 cells with anti-Phospho-YAP1 (S127) antibody (Cat#71227, 1:1,000). Nuclei were stained blue with DAPI; Phospho-YAP1 (S127) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-phospho-YAP1 (S127) antibody (Cat#71227). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-YAP1 (S127) antibody (Cat#71227, 1:20,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-phospho-YAP1 (S127) antibody (Cat#71227). Phospho-YAP1 (S127) expression in wild type (WT) and YAP1 knockout (KO) HSHC cells with 40 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-YAP1 (S127) antibody (Cat#71227, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    YAP1
  • 货号：
    71227
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

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Information
Product Name KO-Validated Anti-Phospho-YAP1 (S127) Rabbit Monoclonal Antibody
Aliases YAP1; Yes1 Associated Transcriptional Regulator; YAP65; YAP-1; Yes-Associated Protein YAP65 Homolog; Transcriptional Coactivator YAP1; Yes Associated Protein 1; Protein Yorkie Homolog; Yes-Associated Protein 1, 65kDa; 65 KDa Yes-Associated Protein; Yes-Associated Protein 2; Yes-Associated Protein 1; Yorkie Homolog; COB1; YAP2; YAP; YKI
Background

Gene Name: YAP1

NCBI Gene Entry: 10413

UniProt Entry: P46937
Application Information

Molecular Weight: Predicted, 54 kDa, observed, 65 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB3280

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human YAP1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western blotting (WB): 1:4,000-1:20,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Phospho-YAP1 (S127) expression in HepG2 cells using anti-Phospho-YAP1 (S127) antibody (Cat#71227, 1:2,000). Green, isotype control; red, Phospho-YAP1 (S127).
  • Immunocytochemical staining of HepG2 cells with anti-Phospho-YAP1 (S127) antibody (Cat#71227, 1:1,000). Nuclei were stained blue with DAPI; Phospho-YAP1 (S127) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-phospho-YAP1 (S127) antibody (Cat#71227). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-YAP1 (S127) antibody (Cat#71227, 1:20,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-phospho-YAP1 (S127) antibody (Cat#71227). Phospho-YAP1 (S127) expression in wild type (WT) and YAP1 knockout (KO) HSHC cells with 40 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-YAP1 (S127) antibody (Cat#71227, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    YAP1
  • 货号：
    71227
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of Phospho-YAP1 (S127) expression in HepG2 cells using anti-Phospho-YAP1 (S127) antibody (Cat#71227, 1:2,000). Green, isotype control; red, Phospho-YAP1 (S127).
Immunocytochemical staining of HepG2 cells with anti-Phospho-YAP1 (S127) antibody (Cat#71227, 1:1,000). Nuclei were stained blue with DAPI; Phospho-YAP1 (S127) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-phospho-YAP1 (S127) antibody (Cat#71227). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-YAP1 (S127) antibody (Cat#71227, 1:20,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-phospho-YAP1 (S127) antibody (Cat#71227). Phospho-YAP1 (S127) expression in wild type (WT) and YAP1 knockout (KO) HSHC cells with 40 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-YAP1 (S127) antibody (Cat#71227, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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