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KO-Validated Anti-YWHAH Mouse Monoclonal Antibody #71262

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Western blotting analysis using anti-YWHAH antibody (Cat#71262). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-YWHAH antibody (Cat#71262, 1:10,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-YWHAH antibody (Cat#71262). YWHAH expression in wild-type (WT) and YWHAH knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-YWHAH antibody (Cat#71262, 1:10,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    YWHAH
  • 货号:
    71262
  • 应用:
    WB
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KO-Validated Anti-YWHAH Mouse Monoclonal Antibody
Aliases YWHAH; Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Eta; YWHA1; Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, Eta Polypeptide; 14-3-3 Protein Eta; 14-3-3 Eta; Protein AS1
Background

NCBI Gene Entry: 7533

UniProt Entry: Q04917

Application Information

Molecular Weight: Predicted MW:28 kDa Observed MW:28 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB3955

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB)

Immunogen Recombinant protein of human14-3-3 eta
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:5,000-1:10000
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-YWHAH antibody (Cat#71262). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-YWHAH antibody (Cat#71262, 1:10,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-YWHAH antibody (Cat#71262). YWHAH expression in wild-type (WT) and YWHAH knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-YWHAH antibody (Cat#71262, 1:10,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Western blotting analysis using anti-YWHAH antibody (Cat#71262). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-YWHAH antibody (Cat#71262, 1:10,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-YWHAH antibody (Cat#71262). YWHAH expression in wild-type (WT) and YWHAH knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-YWHAH antibody (Cat#71262, 1:10,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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