Western blotting analysis using anti-PCSK9 antibody (Cat#71266). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PCSK9 antibody (Cat#71266, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-PCSK9 antibody (Cat#71266). PCSK9 expression in wild type (WT) and PCSK9 knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PCSK9 antibody (Cat#71266, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of PCSK9 expression in HepG2 cells using anti-PCSK9 antibody (Cat#71266, 1：2000). Green, isotype control; red, PCSK9.
Immunocytochemical staining of HepG2 cells with anti-PCSK9 antibody (Cat#71266, 1:1000) . Nuclei were stained blue with DAPI; PCSK9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
