Anti-Phospho-PRAS40 (T246) Rabbit Monoclonal Antibody - 产品中心

Anti-Phospho-PRAS40 (T246) Rabbit Monoclonal Antibody #4666

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Flow cytometric analysis of Phospho-PRAS40 (T246) expression in HeLa cells using anti-Phospho-PRAS40 (T246) antibody (Cat#4666, 1:2,000). Green, isotype control; red, Phospho-PRAS40 (T246).

Immunocytochemical staining of HeLa cells with anti-Phospho-PRAS40 (T246) antibody (Cat#4666, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-PRAS40 (T246) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.

Western blotting analysis using anti-phospho-PRAS40 (T246) antibody (Cat#4666). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-PRAS40 (T246) antibody (Cat#4666, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

AKT1S1
货号：

4666
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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Information

Product Name	Anti-Phospho-PRAS40 (T246) Rabbit Monoclonal Antibody
Aliases	AKT1S1; AKT1 Substrate 1; PRAS40; 40 KDa Proline-Rich AKT Substrate; Proline-Rich AKT1 Substrate 1; MGC2865; Lobe; Proline-Rich Akt Substrate, 40 KDa; AKT1 Substrate 1 (Proline-Rich); AKT1 Substrate 1 (Proline Rich); LOBE
Background	Gene Name: AKT1S1 NCBI Gene Entry: 84335 UniProt Entry: Q96B36
Application Information	Molecular Weight: Predicted, 27 kDa; observed, 40 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB12340 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Phospho-PRAS40 (T246)
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Phospho-PRAS40 (T246) expression in HeLa cells using anti-Phospho-PRAS40 (T246) antibody (Cat#4666, 1:2,000). Green, isotype control; red, Phospho-PRAS40 (T246).
Immunocytochemical staining of HeLa cells with anti-Phospho-PRAS40 (T246) antibody (Cat#4666, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-PRAS40 (T246) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-phospho-PRAS40 (T246) antibody (Cat#4666). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-PRAS40 (T246) antibody (Cat#4666, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

AKT1S1
货号：

4666
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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