Anti-Sirtuin 1 Rabbit Monoclonal Antibody - 产品中心 - 合肥善本生物科技有限公司

Anti-Sirtuin 1 Rabbit Monoclonal Antibody #7367

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Flow cytometric analysis of Sirtuin 1 in HAP-1 cells using Sirtuin 1 antibody (Cat#7367, 1:2,000). Green, isotype control; red, Sirtuin 1.

Immunocytochemical staining of HepG2 cells with Sirtuin 1 antibody (Cat#7367, 1:1,000). Nuclei were stained blue with DAPI; Sirtuin 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using anti-Sirtuin 1 antibody (Cat#7367). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Sirtuin 1 antibody (Cat#7367, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

SIRT1
货号：

7367
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-Sirtuin 1 Rabbit Monoclonal Antibody
Aliases	SIRT1; Sirtuin 1; SIR2L1; NAD-Dependent Protein Deacetylase Sirtuin-1; NAD-Dependent Protein; Deacylase Sirtuin-1; Regulatory Protein SIR2 Homolog 1; SIR2-Like Protein 1; Sirtuin (Silent Mating Type Information Regulation 2, S. Cerevisiae, Homolog) 1; Sirtuin (Silent Mating Type Information Regulation 2 Homolog) 1 (S. Cerevisiae); Sirtuin Type 1; EC 2.3.1.286; EC 2.3.1.-; SIR2alpha; HSIRT1; HSIR2; SIR2
Background	Gene Name: SIRT1 NCBI Gene Entry: 23411 UniProt Entry: Q96EB6
Application Information	Molecular Weight: Predicted, 82 kDa; observed, 120 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB1610 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human SIRT1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Sirtuin 1 in HAP-1 cells using Sirtuin 1 antibody (Cat#7367, 1:2,000). Green, isotype control; red, Sirtuin 1.
Immunocytochemical staining of HepG2 cells with Sirtuin 1 antibody (Cat#7367, 1:1,000). Nuclei were stained blue with DAPI; Sirtuin 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Sirtuin 1 antibody (Cat#7367). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Sirtuin 1 antibody (Cat#7367, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

SIRT1
货号：

7367
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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