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Anti-Alkaline phosphatase Rabbit Monoclonal Antibody #9464

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Flow cytometric analysis of Alkaline phosphatase expression in HeLa cells using Alkaline phosphatase antibody (Cat#9464, 1:2,000). Green, isotype control; red, Alkaline phosphatase.
Immunocytochemical staining of HeLa cells with Alkaline phosphatase antibody (Cat#9464, 1:1,000). Nuclei were stained blue with DAPI; Alkaline phosphatase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Alkaline phosphatase antibody (Cat#9464). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Alkaline phosphatase antibody (Cat#9464, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    ALPL
  • 货号：
    9464
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-Alkaline phosphatase Rabbit Monoclonal Antibody
Aliases Alkaline Phosphatase, Biomineralization Associated; TNSALP; TNALP; TNAP; Alkaline Phosphatase, Tissue-Nonspecific Isozyme; Alkaline Phosphatase Liver/Bone/Kidney Isozyme; Tissue Non-Specific Alkaline Phosphatase; Alkaline Phosphatase, Liver/Bone/Kidney; Phosphocreatine Phosphatase; Phosphoamidase; EC 3.1.3.1; AP-TNAP; TNS-ALP; HOPS; Liver/Bone/Kidney-Type Alkaline Phosphatase; Tissue-Nonspecific ALP; EC 3.9.1.1; APTNAP; HPPA; HPPC; HPPI; HPPO
Background

Gene Name: ALPL

NCBI Gene Entry: 249

UniProt Entry: P05186
Application Information

Molecular Weight: Predicted, 57 kDa; observed, 70 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB1765

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human Alkaline Phosphatase
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,000-1:10,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Alkaline phosphatase expression in HeLa cells using Alkaline phosphatase antibody (Cat#9464, 1:2,000). Green, isotype control; red, Alkaline phosphatase.
  • Immunocytochemical staining of HeLa cells with Alkaline phosphatase antibody (Cat#9464, 1:1,000). Nuclei were stained blue with DAPI; Alkaline phosphatase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-Alkaline phosphatase antibody (Cat#9464). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Alkaline phosphatase antibody (Cat#9464, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    ALPL
  • 货号：
    9464
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Flow cytometric analysis of Alkaline phosphatase expression in HeLa cells using Alkaline phosphatase antibody (Cat#9464, 1:2,000). Green, isotype control; red, Alkaline phosphatase.
Immunocytochemical staining of HeLa cells with Alkaline phosphatase antibody (Cat#9464, 1:1,000). Nuclei were stained blue with DAPI; Alkaline phosphatase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Alkaline phosphatase antibody (Cat#9464). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Alkaline phosphatase antibody (Cat#9464, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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