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Human CLPP Knockdown Cell Line (Wb-Validated) #C1466

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RT-qPCR analysis. HeLa cells were infected with CLPP-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.

Western blotting analysis. CLPP protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies (Cat#68466, 1:5,000) against CLPP and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

CLPP
货号：

C1466
应用：
物种反应性：

规格：

1 Kit

价格：

询价

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Product Name	Human CLPP Knockdown Cell Line (Wb-Validated)
Aliases	CLPP; Caseinolytic Mitochondrial Matrix Peptidase Proteolytic Subunit; ATP-Dependent Clp Protease Proteolytic Subunit, Mitochondrial; Endopeptidase Clp; EC 3.4.21.92; ClpP (Caseinolytic Protease, ATP-Dependent, Proteolytic Subunit, E. Coli) Homolog; ClpP Caseinolytic Peptidase, ATP-Dependent, Proteolytic Subunit Homolog (E. Coli); ClpP Caseinolytic Protease, ATP-Dependent, Proteolytic Subunit Homolog (E. Coli); ClpP Caseinolytic Peptidase, ATP-Dependent, Proteolytic Subunit Homolog; ClpP Caseinolytic Protease, ATP-Dependent, Proteolytic Subunit Homolog; Putative ATP-Dependent Clp Protease Proteolytic Subunit, Mitochondrial; ATP-Dependent Protease ClpAP (E. Coli), Proteolytic Subunit, Human; ClpP Caseinolytic Peptidase ATP-Dependent, Proteolytic Subunit; ATP-Dependent; Protease ClpAP, Proteolytic Subunit, Human; DFNB81; PRLTS3
Background	Gene Name: CLPP NCBI Gene Entry: 8192
Storage	Store at liquid nitrogen for 1 year.
Kit Components	1. Human CLPP Knockdown Cell Line (Wb-Validated) 2. Wild-type cell line
Parental Cell Line	Human cell line supplied by the client
Validation Methods	RT-qPCR, Western blotting (WB)
Shipping	Shipped on Dry Ice.
Instructions For Use	This knockdown cell line should be paired with wild-type cell line for use.
Note	This product is for research use only.

Picture

RT-qPCR analysis. HeLa cells were infected with CLPP-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. CLPP protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies (Cat#68466, 1:5,000) against CLPP and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

CLPP
货号：

C1466
应用：
物种反应性：

规格：

1 Kit

价格：

询价

Datasheet

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